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KMID : 0385520130260040221
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Analytical Science & Technology
2013 Volume.26 No. 4 p.221 ~ p.227
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Validation of LC-MS/MS method for determination of ginsenoside Rg1 in human plasma
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Kim Yun-Jeong
Han Song-Hee
Jeon Ji-Young
Hwang Min-ho
Im Yong-Jin
Lee Sun-Young
Chae Soo-Wan
Kim Min-Gul
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Abstract
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A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the investigation of the ginsenoside Rg1 in human plasma. After addition of internal standard (digoxin), plasma was diluted with acetone and methanol (80:20), the supernatant was concentrated and analyzed by LC-MS/MS. The optimal chromatographic separation was achieved on an Agilent Eclipse XDB-C18 column (4.6 × 150 mm, 5 μm) with a mobile phase of 0.1% formic acid in water and 0.1% formic acid in methanol at a flow rate of 0.9 mL/min gradient mode. The standard calibration curve for ginsenoside Rg1 was linear (r 2 =0.9995) over the concentration range 1~500 ng/mL in human plasma. The intra- and inter-day precision over the concentration range of ginsenoside Rg1 was lower than 7.53% (correlation of variance, CV), and accuracy exceeded 98.28%. This LC-MS/MS assay of ginsenoside Rg1 in human plasma is applicable for quantifying in the pharmacokinetic study.
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KEYWORD
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ginsenoside Rg1, LC-MS/MS, validation, human plasma,
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